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ABC transporters in Dictyostelium discoideum development

Edward Roshan Miranda and Olga Zhuchenko and Marko Toplak and Balaji Santhanam and Blaz Zupan and Adam Kuspa and Gad Shaulsky (2013) ABC transporters in Dictyostelium discoideum development. PLoS One, 8 (8). e70040.

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    ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks.

    Item Type: Article
    Related URLs:
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    http://www.cobiss.si/scripts/cobiss?command=search&base=50070&select=(id=10035284)Alternative location
    Institution: University of Ljubljana
    Department: Faculty of Computer and Information Science
    Divisions: Faculty of Computer and Information Science > Bioinformatics Laboratory
    Item ID: 2260
    Date Deposited: 22 Oct 2013 16:50
    Last Modified: 02 Dec 2013 12:36
    URI: http://eprints.fri.uni-lj.si/id/eprint/2260

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