Nancy Van Driessche and Janez Demsar and Ezgi O Booth and Paul Hill and Peter Juvan and Blaz Zupan and Adam Kuspa and Gad Shaulsky (2005) Epistasis analysis with global transcriptional phenotypes. Nature Genetics, 37 (5). pp. 471-477.
Classical epistasis analysis can determine the order of function of genes in pathways using morphological, biochemical and other phenotypes. It requires knowledge of the pathway's phenotypic output and a variety of experimental expertise and so is unsuitable for genome-scale analysis. Here we used microarray profiles of mutants as phenotypes for epistasis analysis. Considering genes that regulate activity of protein kinase A in Dictyostelium, we identified known and unknown epistatic relationships and reconstructed a genetic network with microarray phenotypes alone. This work shows that microarray data can provide a uniform, quantitative tool for large-scale genetic network analysis.
|Item Type: ||Article|
|Keywords: ||epistasis, gene networks, phenotypes, morphology|
|Related URLs: |
|Institution: ||University of Ljubljana|
|Department: ||Faculty of Computer and Information Science|
|Divisions: ||Faculty of Computer and Information Science > Artificial Intelligence Laboratory|
|Item ID: ||894|
|Date Deposited: ||19 Aug 2009 13:50|
|Last Modified: ||05 Dec 2013 15:22|
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